|
KMID : 1094020110280060571
|
|
Journal of Veterinary Clinics
2011 Volume.28 No. 6 p.571 ~ p.575
|
|
|
Effect of Thawing Rate on the Function of Cryopreserved Canine Sperm
|
|
Kim Su-hee
Kang Tae-Woon
Kim Yong-Jun
Yu Do-Hyeon
|
|
|
Abstract
|
|
|
|
Sperm cryopreservation methods have been improved over the last few decades. However, an optimized thawing rate has not yet been established. Therefore, we investigated the effect of thawing rate on sperm function after cryopreservation. The ejaculates collected from beagle dogs were cryopreserved and then thawed at two different thawing rates (37℃ for 1 min or 70℃ for 15 sec). The thawed sperm were evaluated for motility, viability, morphology, plasma membrane integrity, phosphatidylserine (PS) translocation, and intracellular H₂O₂ level. The sperm thawed rapidly at 70℃ showed improved motility, viability, normal morphology, plasma-membrane integrity and non-PS translocation compared to the sperm thawed slowly at 37℃ (P ＜ 0.05). However, the intracellular H₂O₂ levels were not significantly different between the rapid- and slow-thawed sperm (P ＞ 0.05). In conclusion, sperm rapid thawing at 70℃ could improve the function of cryopreserved canine sperm, and the appropriate thawing rate would enhance the quality of the cryopreserved sperm.
|
|
|
KEYWORD
|
|
|
canine sperm, cryopreservation, motility, thawing rate
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|